周先生和林女士面临双重生育困难：周先生患非梗阻性无精子症，多次精液检查未见精子[1]；林女士则卵巢储备功能严重衰退，AMH仅为0.25ng/ml（正常值2~6ng/ml），卵巢仅剩约4枚卵泡[1]。尽管面临这样的挑战，两人在深圳恒生医院生殖医学科团队的帮助下，通过第二次试管婴儿周期最终实现了二胎梦想，并于2026年6月成功诞下健康宝宝[1]。
医疗团队采用显微取精手术结合激光选精技术，从极少量睾丸组织中找到唯一可用精子[1]。这一过程耗时2小时，胚胎师在手术中现场协作，最终将该精子与林女士仅有的1枚成熟卵子结合[1]。这次取卵培育形成了1枚9细胞一级胚胎，评分为优等[1]。此前，周先生和林女士已于2023年成功生育第一个孩子，当时通过3次取卵获得9枚卵子，形成3枚可用胚胎[1]。本次二胎周期于2025年6月启动，2025年9月进行胚胎移植[1]。
A couple facing extreme reproductive difficulties has successfully achieved a second pregnancy through a combination of cutting-edge assisted reproductive technologies. Mr. Zhou, diagnosed with non-obstructive azoospermia (NOA), and Ms. Lin, whose ovarian reserve function is severely diminished, welcomed a healthy baby in June 2026 after undergoing their second in vitro fertilization cycle at Shenzhen Hengshen Hospital. [1]
Ms. Lin's condition presented a significant challenge, with her anti-Müllerian hormone (AMH) level measuring just 0.25 ng/ml, well below the normal range of 2–6 ng/ml, leaving approximately four follicles remaining in her ovaries. [1] Mr. Zhou's diagnosis of NOA meant that multiple semen analyses had detected no sperm, requiring specialized intervention to retrieve viable reproductive cells. [1] The medical team's breakthrough came through a microsurgical sperm retrieval procedure lasting two hours, during which an embryologist worked on-site to locate a single viable sperm from minimal testicular tissue, combined with laser-assisted sperm selection technology. [1] This single sperm was then used to fertilize the only mature egg obtained during the cycle, resulting in a nine-cell, grade-one embryo—classified as superior quality. [1]
The couple's journey to a second child built upon their earlier success: their first child was born in 2023 after three egg retrieval cycles that yielded nine eggs and three viable embryos. [1] The second IVF cycle began in June 2025, with embryo transfer occurring in September 2025, culminating in the birth of their second healthy child in June 2026. [1]
